FIG. 5.

Knockdown of Pax6 results in increased miR-124 expression in Wt-EBs. (A) Western blot result showing decreased PAX6 expression after knockdown of Pax6 by Ad-Pax6-RNAi infection in Wt-EBs. (B) Knockdown of Pax6 led to elevated miR-124 expression. qPCR analyses were performed independently for EBs (n=5). (C–E) Expression of miR-124 precursors was detected by qPCR. Significant changes were not observed for pre-miR-124-1 (C) or pre-miR-124-2 (D), but pre-miR-124-3 expression (E) was increased in Pax6-knockdown EBs (n=5). A representative experiment is shown; the experiment was repeated three times. Each value represents the mean±SD (**P<0.01). Color images available online at www.liebertpub.com/scd