FIG. 2.

Alterations in microRNA (miRNA) expression profiles in the zebrafish liver after E2 treatment. (A) PCA plot of miRNA microarray results after E2 treatment: each dot represents a chip of the different time points (0, 24, 48 hrs). Performed using Partek-Genomics-Suite software. (B) Volcano plots of miRNA microarray results after E2 treatment at 24 and 48 h. Performed using Partek-Genomics-Suite software. (C) Relative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) results of 20 miRNAs in the male zebrafish liver after E2 treatment at 24 h (mean, n=2) and 48 h (mean±SEM, n=3). Color images available online at www.liebertpub.com/zeb