Effect of binding site mutations in GluK2 or GluK5 on the activation of homomeric and heteromeric receptors by kainate.
A. Representative current traces in response to 5 sec applications of kainate from receptors containing wild-type or mutated subunits as indicated.
B. Concentration-response relationships for kainate were constructed by measuring the peak current amplitude and normalizing this to the maximal peak response to a saturating concentration of kainate for each cell. Symbols represent mean ± SEM (n=4–5 cells) and solid (wild-type) or dashed (mutated) lines show the fit of a four-parameter logistic equation to the averaged data.