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. 2014 Sep 23;12(9):e1001952. doi: 10.1371/journal.pbio.1001952

Figure 3. Rbcn-3α is specifically expressed in gonadotropes in the pituitary.

Figure 3

(A) Double-immunostaining for Rbcn-3α and LH or FSH in the rat pituitary gland showed that Rbcn-3α was expressed in gonadotropes. Scale bars, 10 µm. (B) Double-immunostaining for Rab3GAP or Rab3GEP revealed that these two proteins were present in both LH- and FSH-expressing cells. (C) Immunostaining with antibodies against Rbcn-3α, Rab3GAP, or Rab3GEP and ACTH, TSH, and GH showed that Rbcn-3α, Rab3GAP, and Rab3GEP were not expressed in corticotropes, thyreotropes, and somatotropes. Scale bars, 40 µm. Red staining, antibodies against Rbcn-3α, Rab3GAP, and Rab3GEP. Green staining, antibodies against pituitary hormones.