Table 1.
Variations of X Chromosome Inactivation Status of Female rbESCs
| Undifferentiated d0 | Differentiated d14 | ||||||
|---|---|---|---|---|---|---|---|
| Cell lines (passage) | Xist clouds | H3K27me3 | Relative Xist expression | Xist clouds | H3K27me3 | Relative Xist expression | Class designation |
| B28-6 (P10) | 60% (14/23) | 63% (72/114) | 1.700 | 53% (35/66) | 86% (182/211) | 4.592 | II |
| NJ10 (P8) | 0% (0/20) | 90% (78/86) | 0.214 | 0% (0/22) | 41% (70/167) | 0.037 | III |
| NJ12 (P8) | 44% (22/50) | 93% (280/300) | 0.709 | 51% (17/33) | 87% (114/130) | 1.304 | II |
| P27-1 (P10, male) | 0% (0/30) | 0% (1/112) | 0.0003 | 0% (0/20) | 0% (0/72) | 0.059 | — |
| NJ10-N1 (P10) | 0% (0/28) | 0% (0/92) | 0.112 | 28% (6/21) | 17% (15/86) | 6.611 | I |
| NJ10-N3 (P10) | 0% (0/21) | 0% (0/117) | 0.067 | 56% (18/31) | 35% (33/92) | 12.462 | I |
B28-6, NJ10 and NJ12: female rbESCs; P27-1: male rbESCs. NJ10-N1 and N3 were mESC-like rbESCs converted from NJ10 using four transcription factors.
rbESCs, rabbit embryonic stem cells; mESC-like, mouse embryonic stem cell like.