Table 2.
Differentiation of Female rbESCs In Vitro and In Vivo
| In vitro differentiation | In vivo differentiation | ||||
|---|---|---|---|---|---|
| Class | Cell lines (passage) | EB character | Lineage-specific mark | Teratoma/Injection (SI or RI), size (cm) | Three germ layers |
| II | B28-6 (P10) | Robust | Present | 1/1 (SI), 4.5×2.8 | Present |
| II | NJ12 (P10) | Fair | Present | 1/3 (SI), 4.6×4.0 | Present |
| III | NJ10 (P8-12) | Fair/Robust | Present | 0/3, no teratoma formation by SI. 3/6, 3 teratomas formation by 6 RI. Teratoma #1: 0.4×0.5. Teratoma #2: three of 0.1×0.15. Teratoma #3: 0.2×0.2. |
Endoderm and ectoderm were deficient in all teratomas. Teratoma #1: Primitive and undifferentiated cell mass without any histological structure could be found. Teratoma #2: Contained only cartilage tissues. Teratoma #3: Cartilage tissues covered with some primitive cell mass. |
| — | P27-1 (male, P10) | Fair | Present | 1/2 (SI), 3.0×1.8 | Present |
| I | NJ10-N1 (P8) | Fair | Present | 1/3 (SI), 3.2×3.0 | Present |
| I | NJ10-N3 (P8) | Robust | Present | 2/2 (SI), terotoma #1: 1.8×2.4. terotoma #2: 3.0×4.5 (SI) |
Present |
B28-6, NJ10, and NJ12: female rbESCs; P27-1: male rbESCs. NJ10-N1 and N3 were mESC-like rbESCs converted from NJ10 by four transcription factors.
SI, subcutaneous injection; RI, renal capsule injection.