FIG. 2.

Growth and clonogenicity of ADSCs during the different passages. (A) Proliferative potential of ADSCs obtained from 1 mL of LAs from PM to P3. (B) Cumulative ADSC clonogenicity measured by CFU-F assay from PM to P3. (C) Cumulative time of ADSC culture from both methods and for each passage. (D) Representative example of one colony of CFU-Fs observed from ADSCs obtained at P2 (magnification 50×). (E) Morphology of ADSCs. Representative sample of the typical fibroblastic-like morphology observed after PM (magnification 100×). (D, E) Cells were stained with May-Grünwald/Giemsa solutions. CFU-Fs, colony-forming unit fibroblasts. Color images available online at www.liebertpub.com/scd