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. 2014 May 7;23(19):2390–2400. doi: 10.1089/scd.2014.0071

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 5. — ADSC differentiation into osteoblasts, adipocytes, and chondroblasts. (A) Representative example of ADSC differentiation into osteoblasts after 21 days of induction. Calcium mineralization was assessed by Alizarin Red staining. Left panel, collagenase-treated. Right panel, collagenase-free (magnification 50×). The histograms represent (B) the calcium concentration and (C) the ALP activity determined by colorimetry. n=20. (D) Representative example of ADSC differentiation into adipocytes after 7 days of induction. Left panel, collagenase-treated. Right panel, collagenase-free. Cells were colored using Oil Red O staining (magnification 100×). (E) Histogram represents lipid accumulation determined by colorimetry. n=16. (F) Representative sample of the differentiation into chondrocytes after 21 days of induction. Cell aggregate samples were stained with Alcian blue. Left panel, collagenase-treated. Right panel, collagenase-free (magnification 100×) (n=6). ALP, alkaline phosphatase; CF, collagenase-free; CT, collagenase-treated. Color images available online at www.liebertpub.com/scd