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. 2014 May 7;23(19):2390–2400. doi: 10.1089/scd.2014.0071

FIG. 6.

FIG. 6.

ADSC support of hematopoiesis. (A) Number of Bl-CFCs per 5×103 cord blood CD34+ on ADSC confluent stromal layer obtained with the two methods after 5 and 10 days of incubation in semi-solid media. (Inset) Representative example of refringent Bl-CFC colony observed after 10 days of incubation of cord blood CD34+ cells on a confluent stromal layer of ADSCs (magnification 100×). (B) Number of LTC-ICs per 5×104 cord blood CD34+ cells on ADSC confluent stromal layer obtained with the two methods after 5 weeks of incubation. (C) Number of hematopoietic progenitors (CFUs) from LTC-ICs obtained after 7, 14, and 21 days in semi-solid media with cytokines and EPO. Bl-CFCs: blast-colony forming cells; EPO: erythropoietin; LTC-ICs: long-term culture-initiating cells.