Table 2. BLASTp, Pfam and Clan Matches searched as of May, 2014.
Protein | GenPept accession | Annotations of BLASTp matches | Pfam | Clan |
Predicted Glycosyltransferases | ||||
GtrOC1a | AGK44465 | Hypothetical/Glycosyltransferase | Mito_fiss_Elm1 | CL0113 |
GtrOC2 | AGK44464 | Glycosyltransferase | Glyco_trans_1_2 | CL0113 |
GtrOC3 | AGK44462 | Glycosyltransferase | Glycos_transf_2 | CL0110 |
GtrOC4 | AGK44461 | Glycosyltransferase | Glycos_transf_1, Glyco_transf_4 | CL0113, CL0113 |
GtrOC5 | AGK44459 | Glycosyltransferase | Glyco_transf_25 | CL0110 |
GtrOC6 b | AGK44458 | Hypothetical | n/a | n/a |
GtrOC7 b | AGK44457 | Glycosyltransferase | n/a | n/a |
GtrOC8 | AHK10024 | Glycosyltransferase | Glyco_transf_25 | CL0110 |
GtrOC9 | ABO13302 | Glycosyltransferase | Gly_transf_sug | CL0110 |
GtrOC10 | AHK10023 | Glycosyltransferase | Gly_transf_sug, Gb3_synth | CL0110, n/a |
GtrOC11 | AHK10022 | Glycosyltransferase | Glycos_transf_1 | CL0113 |
GtrOC12 | EPG40646 | Glycosyltransferase | Glyco_transf_4, Glycos_transf_1 | CL0113, CL0113 |
GtrOC13 | AHK10235 | Glycosyltransferase | Glyco_transf_4, Glycos_transf_1 | CL0113, CL0113 |
GtrOC14 | AHK10234 | Hypothetical | Mito_fiss_Elm1 | CL0113 |
GtrOC15 | AHK10233 | Glycosyltransferase | Glyco_trans_4_2, Glycos_transf_1 | CL0113, CL0113 |
GtrOC16 | AHK10230 | Glycosyltransferase | Glyco_transf_8, Glyco_transf_8C | CL0110, n/a |
GtrOC17 | AHK10229 | Glycosyltransferase | Glyco_trans_1_2 | CL0113 |
GtrOC18 | EKU52820 | Glycosyltransferase | Glyco_transf_4, Glycos_transf_1 | CL0113, CL0113 |
GtrOC19a | EKU52938 | Hypothetical/Glycosyltransferase | Mito_fiss_Elm1 | CL0113 |
GtrOC19b | EKP44745 | Hypothetical/Glycosyltransferase | Mito_fiss_Elm1 | CL0113 |
GtrOC19c | EKK07863 | Hypothetical/Glycosyltransferase | Mito_fiss_Elm1 | CL0113 |
GtrOC20 | EKU52965 | Glycosyltransferase | Glycos_transf_2 | CL0110 |
GtrOC21 | EKU52883 | Hypothetical/Glycosyltransferase | Glyco_trans_1_2, DUF3880 | CL0113, n/a |
GtrOC22 | EKP65514 | Hypothetical/Glycosyltransferase | Mito_fiss_Elm1 | CL0113 |
GtrOC23 | EKP65507 | Glycosyltransferase | Glyco_trans_4_2 Glycos_transf_1 | CL0113CL0113 |
GtrOC24 | EKP65575 | Glycosyltransferase | Glycos_transf_2 | CL0110 |
GtrOC25 | EKP65473 | Glycosyltransferase | Glycos_transf_1 | CL0113 |
GtrOC26 | EKP44834 | Glycosyltransferase | Glycos_transf_2 | CL0110 |
GtrOC27 | EKK07911 | mannosyltransferase | Caps_synth | CL0110 |
GtrOC28 | EKK07913 | Glycosyltransferase | Glyco_tranf_2_5 | CL0110 |
GtrOC29 | WP_000760350 | Glycosyltransferase | Glyco_transf_25 | CL0110 |
GtrOC30 | WP_000760349 | Glycosyltransferase | Glyco_transf_25 | CL0110 |
GtrOC31 | WP_024432893 | Glycosyltransferase | Glyco_transf_25 | CL0110 |
Other | ||||
Ahy | EKP65391 | GDSL-like lipase/acylhydrolase family protein | Lipase_GDSL_2 | CL0264 |
AtrOC1 | AHK10232 | Acyltransferase | Acyl_transf_3 | CL0316 |
Glf | EPG40651 | Glf, UDP-galactopyranose mutase | GLF, NAD_binding_8 | CL0063, CL0063 |
(HtrL) | AHK10231 | HtrL/YibB protein | HtrL_YibB | n/a |
Orf1 c | AGK44460 | Hypothetical protein | n/a | n/a |
Orf2 | EKP65421 | Hypothetical | n/a | n/a |
Orf3 | WP_000793090 | Hypothetical | DUF707 | n/a |
Pda1 | AGK44463 | Polysaccharide deaceylase | Polysacc_deac_1 | CL0158 |
Pda2 | EKP44902 | Polysaccharide deaceylase | Polysacc_deac_1 | CL0158 |
Pda2a | AHK10236 | Polysaccharide deaceylase | Polysacc_deac_1 | CL0158 |
PtrOC1 | WP_024432894 | Glycosyltransferase/pyruvyltransferase/exosortase | PS_pyruv_trans | CL0113 |
RmlA | EKU52850 | RmlA, glucose-1-phosphate thymidylyltransferase | NTP_transferase | CL0110 |
RmlB | EKU52900 | RmlB, dTDP-glucose 4,6-dehydratase | Epimerase | CL0063 |
RmlC | EKU52972 | RmlC, dTDP-4-dehydrorhamnose 3,5-epimerase | dTDP_sugar_isom | CL0029 |
RmlD | EKU52914 | RmlD, dTDP-4-dehydrorhamnose reductase | RmlD_sub_bind | CL0063 |
WecB | ABO13303 | UDP-N-acetylglucosamine 2-epimerase | Epimerase_2 | CL0113 |
GtrOC1 predicted as a glycosyltransferase as it belongs to Mito_fiss_Elm1 protein family, similar to other GtrOC proteins listed. BLASTp matches hit sequences incorrectly annotated as nucleotide-diphosphate sugar epimerases.
GtrOC6 and GtrOC7 predicted as glycosyltransferases given that 7 glycosyltransferases are required for construction of OC1 [12]. Preliminary experimental data supports GtrOC6 prediction [16].
searches conducted in September, 2012 predicted that Ghy belonging to Pfam Glyco_hydro_43 (CL0143) and ghy was used in [12]. First BLASTp hit outside of Acinetobacter is a putative formyl transferase in Candidatus Nitrosoarchaeum koreensis [GenPept accession number WP_007549433] (78% coverage, 37% identity), which also has no predicted Pfam. Gene name has been changed to orf1 according to updated searches as of May, 2014.