Skip to main content
. Author manuscript; available in PMC: 2014 Sep 24.
Published in final edited form as: J Biomol Screen. 2011 Oct 27;17(2):163–176. doi: 10.1177/1087057111423417

Figure 8. NIH chemical library HTS screen data.

Figure 8

(A) Shown are Z' values per plate from the primary HTS assay performed in a 1536-well format using the NIH small molecule library [N ≈ 330,000 compounds]. The x-axis represents the assay plate number vs. the y-axis showing the Z'. The average Z' factor observed was 0.78. (B) The primary HTS screen is represented in Histogram format. The x-axis represents % activity vs. the y-axis, which corresponds to the number of compounds. For inhibitors, a hit cutoff of >= 45 % activity (compared to the controls) was chosen, representing two standard deviations from the mean. (C) The primary HTS screen is also represented in Scatterplot format. The x-axis represents SourcePlate ID (each plate representing a subset of chemical compounds) vs the y-axis, which corresponds to the % Activity. For inhibitors, a hit cutoff of ≥ 45 % activity (compared to the controls) was chosen, representing two standard deviations from the mean.