. 2014 Sep 23;9(9):e108022. doi: 10.1371/journal.pone.0108022

Table 1. Comparetive binding parameters for the interaction of harmalol with polynucleotides in 15 mM CP buffer at 25±0.5°C obtained from spectrophotometric and spectrofluorimetric studies^a.

Polynucleotides	Spectrophotometry (analysis by McGhee-von Hippel)						Spectrofluorimetry φ/φ_o ^b	Thermal melting ΔT_m (°C)
	Binding constant K_i (×10⁶ M⁻¹)	n	Cooperativity factor (ω)	K_iω (×10⁶M⁻¹)	Binding mode	Hypochromic effect (%); bathochromic shift (nm)
poly(dA-dT).poly(dA-dT)	0.43±0.03	0.18	-	-	Non cooperative	33%; 6 nm	1.43	8
poly(dA).poly(dT)	0.02±0.01	0.12	14.0	0.28±0.01	cooperative	21.88%; 4 nm	1.20	6
poly(dG-dC).poly(dG-dC)	4.20±0.05	0.33	-	-	Non cooperative	44%; 13 nm	0.20	ND
poly(dG).poly(dC)	0.002±0.001	0.08	84	0.18±0.01	cooperative	31.36%; 5 nm	0.56	6

ND; not determined.

Data presented from the average of four determinations in each. Denotes the relative quantum yield value at saturation of nucleotide/alkaloid molar ratio determined as described (ref). ΔT_m °C is calculated [T_m of complex - T_m of duplex polynucleotide] at r_max.

Table 1. Comparetive binding parameters for the interaction of harmalol with polynucleotides in 15 mM CP buffer at 25±0.5°C obtained from spectrophotometric and spectrofluorimetric studiesa.

Table 1. Comparetive binding parameters for the interaction of harmalol with polynucleotides in 15 mM CP buffer at 25±0.5°C obtained from spectrophotometric and spectrofluorimetric studies^a.