Table 3. Temperature dependent thermodynamic parameters for the binding of harmalol to the hetero polynucleotides.

Polynucleotides	Temperature (K)	K b (×106 M−1)	ΔGo kcal/mol	ΔHo kcal/mol	TΔSo kcal/mol	ΔCp o kcal/mol K	ΔGhyd kcal/mol
poly(dG-dC).poly(dG-dC)	288.15	4.70±0.47	−8.85	−3.8	5.05
	293.15	4.20±0.27	−8.94	−4.30	4.64	−0.150	−12.0
	298.15	3.86±0.07	−9.04	−5.00	4.04
	303.15	2.96±0.06	−9.03	−6.07	2.96
poly(dA-dT).poly(dA-dT)	288.15	0.52±0.09	−7.58	−0.61	6.97
	293.15	0.45±0.07	−7.63	−0.87	6.76	−0.118	−9.4
	298.15	0.44±0.02	−7.74	−2.00	5.74
	303.15	0.31±0.01	−7.66	−2.20	5.46

All the data in this table are derived from ITC experiments conducted in 15 mM CP buffer, pH 6.8 and are average of three determinations. Kb and ΔH o values were determined from ITC profiles fitting to Origin 7 software as described in the text. The values of ΔGo and TΔSo were determined using the equations ΔGo = −RTlnKb and TΔSo = ΔHo−ΔGo. All the ITC profiles were fit to a model of single binding sites.