Figure 3. Effect of G-MDSC on IFN-gamma production.

(A) Cord blood CD3^pos T cells and adult CD3^pos CD45RO^neg T cells were assessed for IFN-gamma production by ELISpot after anti-CD3/CD28 bead stimulation (n = 11 independent experiments performed in triplicate for neonates and 9 independent experiments for adults). Statistical significance determined by the Mann Whitney test. (B) Neonatal G-MDSC decrease IFN-gamma production after anti-CD3/CD28 bead stimulation. (n = 13 independent experiments performed in triplicate). (C) G-MDSC frequency correlation with suppression T cell of IFN-gamma production by G-MDSC. G-MDSC frequencies were correlated to suppression using the Spearman rank correlation test (n = 11 independent experiments).