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. 2014 Sep 23;9(9):e107776. doi: 10.1371/journal.pone.0107776

Figure 4. DMH1 has no cytotoxicity, but inhibited SDH activity and ATP production.

Figure 4

(A) DMH1 did not increase LDH release in L6 cells. Cells were incubated with 1 µM, 5 µM and 10 µM DMH1 for 24 hrs. Then LDH units were normalized to total protein content. n = 5. (B) Representative photographs showing that DMH1 did not induce cell death. Cells were treated with 10 µM DMH1 for 24 hrs. The labeled cells were photographed under a fluorescence microscope. The live cells fluoresce green and dead cells fluoresce red. (C) MTT results showed that DMH1 inhibited succinate dehydrogenase (SDH) activity. n = 24. **P<0.01 vs control. (D) DMH1 treatment reduced ATP production in L6 cells. n = 6. **P<0.01 vs control.