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. 2014 Sep 23;9(9):e108066. doi: 10.1371/journal.pone.0108066

Figure 1. CHD5 co-purifies with the NuRD transcriptional repressor complex.

Figure 1

(A) FLAG-tagged CHD4 and CHD5 were immunoprecipitated from a transiently transfected HEK293T-derived cell line, and the samples were analyzed by western blotting using the indicated antibodies. The empty vector was also transfected in parallel [Control]. (B) The HEK293T-derived line was transiently transfected with increasing amounts of the CHD5-expression plasmid. An empty vector was included where necessary to keep the final amount (15 µg) of transfected plasmid constant. Following transfection, cell extracts and RNA were prepared to measure CHD4 levels by western blotting and CHD4 and CHD5 mRNA levels by qRT-PCR.