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. 2014 Sep 23;9(9):e108066. doi: 10.1371/journal.pone.0108066

Figure 2. CHD5 expression leads to repression of WEE1.

Figure 2

(A) The neuroblastoma line KELLY or (B) 293T-derived cells were transiently transfected with vector (vec.) or the vector containing the human CHD5 cDNA. In the case of the 293T-derived cells, two amounts of CHD5 cDNA were transfected (see Methods). Two days after transfection, the cells were harvested and total RNA was prepared. Quantitative RT-PCR was performed to monitor the levels of the CHD5 and WEE1 transcripts. The values shown are normalized to a 5S rRNA housekeeping transcript. (C) CHD5 levels were reduced siRNA-mediated knockdown in PANC-1 cells. As a control (ctrl), a scramble siRNA was transfected in parallel. The levels of CHD5 protein were detected by western blotting (left) and the levels of WEE1 mRNA were analyzed by qRT-PCR. Error bars represent SD [n = 3].