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. 2007 Jun 14;13(22):3071–3079. doi: 10.3748/wjg.v13.i22.3071

Figure 1.

Figure 1

Effect of recombinant MLN64 adenovirus infection on MLN64 expression in mice livers. A: Immunoblot analysis of MLN64. At 24 h after infection, mitochondria/lysosome enriched extracts (50 μg protein/lane from Ad.E1Δ and 1 μg protein/lane from Ad.MLN64 mouse livers) were subjected to 10% SDS-PAGE and Western blotting with anti-MLN64 and anti-ε-COP antibodies; B: Time course of hepatic MLN64 expression after administration of recombinant adenovirus. At the indicated times (in hours) after infection, livers were collected and analyzed for MLN64 protein expression in total membrane extracts as described in (A); C: Immunofluorescence analysis of liver tissue 24 h after infection with control (Ad.E1Δ) or MLN64 (Ad.MLN64) recombinant adenovirus. Bar: 10 μm. Analysis were performed in four mice in each experimental group. Results are representative of three independent experiments.