Effect of MLN64 over expression on apoptosis in CHO cells. CHO-K1 cells were transfected with a pcDNA3.1 empty or a pcDNA3.1/MLN64 vector, analyzed by MLN64 immunofluorescence (A, E), TUNEL (B, F), or Hoechst 33258 (C, G) staining, and visualized under fluorescence microscopy. Merge of panels A, B and C and E, F and G are shown in panels D and H. Arrows indicate MLN64 overexpressing cells.