Figure 6.

Effect of a 2% CDCA diet on hepatic MLN64 mRNA and protein levels in mice. Mice were fed with a 2% CDCA diet for 1 or 2 d. A: Real time PCR was used to analyze mln64 RNA expression. Each value represents the mean ± SE for mRNA values determined in CDCA-fed mice relative to those measured in chow diet-fed mice. There were 5 mice in each group. ^bP < 0.0001; B: Immunoblot analysis of MLN64. Mitochondria-enriched liver extracts (50 μg protein/lane) were subjected to 10% SDS-PAGE and Western blotting with anti-MLN64 and anti-ε-COP antibodies. A liver membrane fraction from an Ad.MLN64-infected mouse was used as a positive control (C+). ^aP < 0.05.