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. 2014 Sep 23;9(9):e107764. doi: 10.1371/journal.pone.0107764

Figure 1. rCSP Strain Selection and Identification.

Figure 1

(A) Reduced SDS-PAGE analysis of lysate samples prior to purification. Lanes 1–3 represent samples from strain CSP-1, CSP-3, and CSP-2 fermentations, respectively. Lane 4 represents a sample from a culture of P. fluorescens transformed with plasmid vector containing no insert. Arrow denotes rCSP in lysate samples. (B) Reduced SDS-PAGE analysis of purified proteins from a 2-column purification process. Lanes 1–3 represent samples from CSP-1, CSP-2, and CSP-3 productions, respectively. (C) Western blot of non-reduced purified CSP-1, CSP-2, and CSP-3 proteins (lanes 2–4, respectively). Lane 1 represents a sample from a culture of P. fluorescens transformed with plasmid vector containing no insert.