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. 2014 Sep 23;9(9):e108424. doi: 10.1371/journal.pone.0108424

Figure 2. Major parameters to identify gRNA-Cas9 target sites that are shared or distinct in two related sequences with the compare2sequences function of CRISPRseek.

Figure 2

compare2sequences first identifies target sites in two input sequences using many of the same parameters available for the offTargetSequence function. Next, cleavage scores are determined for each target site in both target sequences.