Figure 1. Representive Western blot (A, D) and densitometric quantification of eNOS uncoupling (B, E) and phospho-p66^Shc protein (C, F) expression in HAEC treated with oxLDL within sixty minutes (A, B, C) or for twenty four hours (D, E, F).

eNOS uncoupling was presented as ratio of dimer/monomer form of eNOS. The phosphorylation of p66^Shc was normalized to total p66^Shc protein and total p66^Shc was normalized to α-tubulin. Results are presented as means±SEM; n = 6. * p<0.05 vs. cells at 0 minutes or cells under control condition.