Figure 5. Representive Western blot (A) and densitometric quantification of eNOS uncoupling (B) and phospho-p66^Shc protein (C) in HAEC after twenty-four hours incubation with oxLDL in the presence of tetrahydrobiopterin [BH4 10 µM; before (B), forty five minutes after (After Early, AE), or sixteen hours after (After Later, AL) oxLDL treatment].

eNOS uncoupling was presented as ratio of dimer/monomer form of eNOS. The phosphorylation of p66^Shc was normalized to total p66^Shc protein and total p66^Shc was normalized to α-tubulin. Results are presented as means±SEM; n = 8. * p<0.05 vs. cells treated with oxLDL alone.