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. 2014 Sep 23;9(9):e108506. doi: 10.1371/journal.pone.0108506

Figure 1. Genomic organization of the Japanese Crested Ibis MHC class II region.

Figure 1

Three contigs representing different haplotypes were constructed. Collagen-type XI α-2 (COL11A2), MHC-IIA (α), MHC-IIB (β) and bromodomain-containing 2 (BRD2) genes and their orientations are indicated. Locus names are indicated below the MHC-IIA and -IIB genes. The types of MHC-IIB exon 2 sequences (Table 1) are shown below the locus names. B, K, P, S, Sc, and X represent restriction sites used for subcloning of BamHI, KpnI, PstI, SalI, SacI, and XhoI, respectively. Solid bars below the map represent locations of isolated lambda phage clones. The bidirectional arrow above DAB2 in haplotype 3 indicates the first amplified MHC-IIB fragment.