Table 2.
Primary antibodies used in the present study
| Antibody | Host | Dilution | Source |
|---|---|---|---|
| TPPP (C-ps B) | rabbit | 1:500 (IHC, IF) | our laboratory |
| 1:20,000 (WB) | |||
| 1:20 (IEM) | |||
| TPPP (N-mab#2A8G3) | rat | 1:50 (IHC, IF) | our laboratory |
| 1:2000 (WB) | |||
| 1:20 (IEM) | |||
| GFAP | mouse | 1:500 (IF) | Sigma |
| IBA1 | rabbit | 1:500 (IF) | Proteintech |
| MBP | rabbit | 1:500 (IF) | DAKO |
| OLIG2 | rabbit | 1μg/ml (IF) | IBL |
| actin | rabbit | 1:20,000 (WB) | Sigma |
| lamin B1 | rabbit | 1:500 (IF) | Santa Cruz |
| β-tubulin | mouse | 1:5000 (WB) | BD Pharmingen |
| TOM20 | rabbit | 1:100 (IF) | Santa Cruz |
| 1:3000 (WB) | |||
| calnexin | rabbit | 1:20,000 | Proteintech |
| histone H3 | rabbit | 1:2000 (WB) | Cell Signaling |
| cytochrome C | mouse | 1:200 (IF) | BD Pharmingen |
| DRP1 | rabbit | 1:200 (IHC, IF) | Abcam |
| MFN2 | rabbit | 1:100 (IHC) | Proteintech |
| pα-syn | mouse | 1:4000 (IHC,IF) | WAKO |
IHC: immnohistochemistry; IF: immunofluorescence.WB: western blotting; IEM: immunoelectron microscopy.