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. 2014 Sep 18;14:189. doi: 10.1186/s12862-014-0189-9

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© Darwell et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Output from jMOTU suggests five P. imperialis MOTUs according to percentage sequence discrimination threshold employed for cytb sequences. Plateau between 17–30 bp indicates barcoding gap.