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Tbr2 is essential for the formation of M1–M3 ipRGCs. Confocal images of control Tbr2flox/+ (A) and mutant Six3–Cre:Tbr2flox/flox (B) flat-mounted retinas, immunolabeled with anti-melanopsin/Opn4 antibody at P30. The central retina in B shows no Opn4+ cells, which is consistent with the presence of the LacZ reporter in the Six3–Cre:Tbr2flox/flox:Opn4Tau–LacZ/+ retina shown in Figure 2. OD: Optic disc.
