Figure 1.

NB dye coupling of TRN neurons. A, Staining of cells, blood vessels, and extracellular matrix by NB. Image of a thalamic slice with two TRN neurons injected with NB-containing internal solution through whole-cell patch pipettes. Pipettes were filled only with NB (0.5%)-containing internal solution (no front-filling). Blue arrows indicate injected neurons. B, An example of a dye-coupled cluster from an NB-injected TRN neuron from a rat TC slice. The blue arrow indicates the injected cell. Low magnification is on the right, and high magnification is on the left. dp, Dorsoposterior; la, latero-anterior. C, Another example of a dye-coupled cluster from a rat TC slice. D, Left, Diagram of cutting angles for TC and Hz slices. Right, Schematics of the shapes of TC and Hz slices and the orientation of the TRN and VB in each slice. x and y axes were used for measuring coordinates of dye-coupled neurons. a, Anterior; d, dorsal; dp, dorsoposterior; HP, hippocampus; l, lateral; la, latero-anterior; m, medial; p, posterior; v, ventral.