Figure 4.

GST-Thorase pull-down of GluR2, GRIP1, Nicalin, and NSF from whole mouse brain lysates. A, Whole-brain lysates were mixed with GST beads prebound to purified recombinant GST-tagged wild-type (WT), N-terminal truncated (N100), and C-terminal truncated (C20) Thorase in the presence of ATPγS. Input lanes are in the absence of lysate. The experiments were repeated three times with similar results. B, Optical densitometry quantification of pull down lanes from the blots in A with values representing the percent compared to Lysate lane, mean ± SEM (n = 3, **p < 0.001, *p < 0.05, n.s. p > 0.05, one-way ANOVA, post-test: Tukey's multiple comparison).