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. 2014 Sep 16;14:239. doi: 10.1186/s12866-014-0239-x

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© Tillich et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Cultivation over 13 days with daily dilutions back to the starting OD of 0.4. The cell count at the various wells (edge of the plate (green) & middle wells (blue)) are shown over the cultivation time. Light intensity was varied during the cultivation time [photon flux in μmol m^-2 s^-1]: Day 1-3: 15; Day 4-7: 32; Day 8-10: 51; Day 11-13: 72.