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. 2014 Sep 10;14:238. doi: 10.1186/s12866-014-0238-y

Figure 1.

Figure 1

Growth comparison between defined (HX25M and AR-103) and complex (LITB+FBS and LITB without FBS) media. Epimastigote cells counts were performed every three days, then all cultures were diluted to 1e + 06 cells/ml (start point of cultivation). Values plotted refer to the average of three biological replicates counted every cell passage after three days of cultivation. Cell cultivation in LITB+FBS (black line) and LITB without FBS (dashed line) was maintained for five passages of three days, whereas defined media HX25M (red line) and AR-103 (green line) did not support cell growth after the third passage.