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Expression of N-terminally truncated ST6Gal-I variants in P.pastoris KM71H in the absence (−PI) and presence (+PI) of protease inhibitors. His-tagged enzyme variants were expressed for 96 h with (+PI) and without (−PI) protease inhibitor. WB analysis of fermentation supernatants (each 600 μL, precipitated with 20% TCA). Immunodetection with HRP conjugated anti-α2,6ST6.
