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. 2014 Sep 11;13:138. doi: 10.1186/s12934-014-0138-8

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© Ribitsch et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Schematic representation of the pPICZαB-based P.pastoris expression vectors employed in this study. pAOX1: alcohol oxidase 1 gene promoter; α-factor: coding region for the signal sequence of S. cerevisae α-mating factor; 6xHis: coding sequence for 6 histidines; Flag: coding sequence for the Flag-Tag; Kex2: coding sequence for Kex2 protease cleavage sites; human ST6Gal-I: coding sequence for the truncated variants of human ST6Gal-I.