Table 3.
Primary and secondary antibodies
| Experiments | Primary antibodies | Host | Dilution | Source |
|---|---|---|---|---|
| Quantitative immunostaining | Synaptophysin | Mouse | 1:500 | Millipore |
| GAD65 | Rabbit | 1:500 | Millipore | |
| Western blot | NR2B | Rabbit | 1:6000 | Santa Cruz |
| pCamkII (THr286) | Rabbit | 1:1000 | Cell Signaling | |
| CamkII | Rabbit | 1:1000 | Cell Signaling | |
| pCreb (Ser133) | Rabbit | 1:50 | Cell Signaling | |
| Creb | Rabbit | 1:1000 | Cell Signaling | |
| GAPDH | Rabbit | 1:6000 | Cell Signaling | |
| BACE1 | Rabbit | 1:500 | Cell Signaling | |
| 6E10 (β-CTF) | mouse | 1:6000 | Signet | |
| hsAPPβ | Rabbit | 1:50 | IBL | |
| Experiments | Secondary antibodies | Dilution | Source | |
| Quantitative immunostaining | CF™488 Conjugated Goat anti-Mouse IgG(H + L) | 1:200 | Biotium | |
| CF™488 Conjugated Goat anti-Rabbit IgG(H + L) | 1:200 | Biotium | ||
| Western blot | HRP-conjugated Goat anti-Rabbit IgG(H + L) | 1:20000 | ZSGB-BIO | |
| HRP-conjugated Goat anti-Mouse IgG(H + L) | 1:25000 | CST | ||