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Globular domain gain/loss as a function of the variation between the four human (Rspo)s. The disorder propensity of the protein stretch was calculated using GlobPlot analyses to identify the disorder region (blue). The upper portion in the figure illustrates the differences between the amino acid sequence alignments among the domains. The tool uses a simple peak-finder algorithm to select the putative globular and disorder segments. (a) Rspo1, (b) Rspo2, (c) Rspo3, and (d) Rspo4.
