Table 2.
Primer sequences used for qualitative and quantitative (q) real time RT-PCR, and RACE and the expected product size
| Primer name | Assay | Sequence (5′-3′) | Exon position | Target gene | Amplicon sizes (bp) |
|---|---|---|---|---|---|
| GHRL-1 F | RT-PCR | TTTCTGAGCCCTGAACATCAG | 1 | GHRL | 305 canonical (wild-type) preproghrelin |
| GHRL-4R | GAGAACAGACAGGTGGTTGG | 4 | |||
| GHRL-5OF | RACE | ATGAATGAACACTGCGTTTGCT | N/A | GHRL | 174 |
| GHRL-5OR | CAGTTTCTGATGTTCAGGGCTC | 1 | |||
| GHRL-5IF | RACE | GAACACTGCGTTTGCTGGCT | N/A | GHRL | 130 |
| GHRL-5IR | GGCCAAGTCCATCCAGAGCA | 1 | |||
| GHRL-2F | qRT-PCR | CTAAGAAGCCGTCAGGCAGACT | 2 | GHRL (preproghrelin) | 153 |
| GHRL-2R | GGGACTGAGCCCCTGACA | 3 | |||
| GHRL1_3F | qRT-PCR | CAGAAACTGCAGTTCAATGC | 1 / 3 | GHRL (Δex2 preproghrelin) | 224 |
| GHRL-4R | CACGTGGTCTCGGAAGTGT | 4 | |||
| GHRL1_4F | qRT-PCR | CTGCAGAAACCCTGGCTGA | 1 / 4 | GHRL (Δex2,3 preproghrelin) | 109 |
| GHRL-4R | CACGTGGTCTCGGAAGTGT | 4 | |||
| GOAT-mF | qRT-PCR | GGTTTCAAGCTCGAGTTGAAGG | N/A | MBOAT4 (GOAT) | 212 |
| GOAT-mR | AGTAGGTGAGTTTGAAGAGCCC | ||||
| GAPDH-F | qRT-PCR | CCTGGAGAAACCTGCCAAGT | N/A | GAPDH | 209 |
| GAPDH-R | GCCAAATTCATTGTCGTACCA |