Table 2.
The strains and plasmids used in this study
| Strain or plasmids | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | Host for general cloning | TransGen Biotech |
| E. coli TOP10 | Host for general cloning | TransGen Biotech |
| E. coli S17-1 | Donor stain for conjugation between E. coli and S. spinosa | [25] |
| S. spinosa ATCC 49460 | Wild strain | [26] |
| S. spinosa Lu106 | S. spinosa ATCC 4946 with pLu106 | This study |
| Plasmids | ||
| POJ260 | E. coli – Streptomcyes shuttle vector; apr oriT rep PUC lacZ | [27] |
| pLu106 | pOJ260 with truncated Rex | This study |