Figure 5.
P. pachyrhizi development on the detached leaves at 12 days after inoculation. Three detached leaves of each one transgenic line and two wild-type plants were inoculated with 105/mL spore suspension and incubated at 20°C. (A) Two infection parameters were evaluated: number of lesions and number of pustules. *Means are significantly different in leaves of wild type (wt) and transgenic soybean line P3-2. (Student’s t-test, p < 0.05). (B) Low number of tan-colored lesions and pustules under stereomicroscope in a leaf of wild-type (wt) plant. (C) High number of tan-colored lesions and pustules under stereomicroscope in a leaf of transgenic soybean line P3-2 with suppression of the four WRKYs.