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. 2014 Sep 10;14:236. doi: 10.1186/s12870-014-0236-0

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© Bencke-Malato et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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T-DNA region of binary vectors used for Gm WRKY27 overexpression or Gm WRKY silence in soybean. (A) Overexpression construct - pH7WG2D.1-GmWRKY27. The full-length ORF of GmWRKY27 was cloned in the vector. (B) RNAi suppression construct - pH7GWIWG2(II).0-GmWRKY. Inverted repeats of a 176-bp WRKY fragment was cloned into the vector. RB – T-DNA right border, LB – left border, hpt – hygromycin phosphotransferase gene, P35S – Cauliflower mosaic virus (CaMV) 35S promoter, T35S – CaMV 35S terminator, EgfpER – enhanced green fluorescent protein, ProlD – root loci D promoter, WRKY – soybean 176 pb WRKY fragment, attB1 and attB2 – LR reaction site.