Table 4.
Primer set designed for RT-qPCR
| Target | Orientation | Tm (°C)* | Primer Sequence | PCR product size (pb) |
|---|---|---|---|---|
| GmWRKY20 transcripts | Forward | 60 | 5′-TTGCAAAGTTCAGAAGTATCTTGTC-3′ | 264 |
| Reverse | 60 | 5′-GTGACCTGTTGTAGATCCCATC-3′ | ||
| GmWRKY27 transcripts | Forward | 61.69 | 5′-GATTGTGCATTTGCTAATCATGC-3′ | 105 |
| Reverse | 59.93 | 5′- GCTATAGAAACTTCGCCAGAAC-3′ | ||
| GmWRKY46 transcripts | Reverse | 60 | 5′-CAATGCATCATCAACTTCCG-3′ | 213 |
| Forward | 60 | 5′-CAAGACCACTTTCACAGCTCAC-3′ | ||
| GmWRKY56 transcripts | Forward | 60.06 | 5′-CACCCATCTGCCTCATCAC-3′ | 234 |
| Reverse | 59.17 | 5′-GGAGGCCGAGTCTGTACAAT-3′ | ||
| GmWRKY57 transcripts | Forward | 60 | 5′-TCCCTCAACTTCCTCCAATC-3′ | 170 |
| Reverse | 60 | 5′-GGAAGGGTTCAAAGGCATC-3′ | ||
| GmWRKY106 transcripts | Forward | 59.72 | 5′-GGAAATAAAGTTCCACTAAGGATGAC-3′ | 174 |
| Reverse | 60.57 | 5′ - CCGAGAATGTGTGCTACAACC-3′ | ||
| GmWRKY125 transcripts | Forward | 60 | 5′-TCTCATCTTCCAATAATTTCCCA-3′ | 135 |
| Reverse | 60 | 5′-CATGATGCCTTGGTGAGCTA-3′ | ||
| GmWRKY139 transcripts | Forward | 60.53 | 5′-CAAATCCTTTTGGTGGGAATC-3′ | 145 |
| Reverse | 59.30 | 5′-CTATAGAAATTTCGCAAGAACTTAACC-3′ | ||
| Metalloprotease transcripts | Forward | 60.5 | 5′-ATGAATGACGGTTCCCATGTA-3′ | 114 |
| Reverse | 60.17 | 5′-GGCATTAAGGCAGCTCACTCT-3′ | ||
| FBox transcripts | Forward | 60.25 | 5′-AGATAGGGAAATGTTGCAGGT-3′ | 93 |
| Reverse | 59.84 | 5′-CTAATGGCAATTGCAGCTCTC-3′ |
*Calculated Tm under PCR conditions.