Table 5.
Primer set designed to gene isolation and transgene detection
| Target | Orientation | Tm (°C) | Primer Sequence | PCR product size (pb) |
|---|---|---|---|---|
| 35S | Forward | 52 | 5′-GGACCCCCACCCACGAGGAG-3′ | 139* |
| GmWRKY27 overexpression | Forward | 58 | 5′- CACCATGGATTATTCATCATGGATTAACA-3′ | 921 |
| Reverse | 5′- TTAATTATTATTGTGCAACATTTTTC-3′ | |||
| WRKY (RNAi) | Forward | 58 | 5′- CACCCTTCCTTGGATCTCAACATTAATCT -3′ | 176 |
| Reverse | 5′- TAACTTCTTGTTTTCTGCACTCACC -3′ | |||
| Intron | Forward | 60 | 5′- TGCCTCTTCTTACGGCTTTCTGTG -3′ | 400 |
| Reverse | 5′ - TGCCGTCTGTGATGGCTTCCA -3′ | |||
| Hpt | Forward | 60 | 5′-GCGATTGCTGATCCCCATGTGTGTAT-3′ | 512 |
| Reverse | 5′-GGTTTCCACTATCGGCGAGTACTT-3′ |
*Fragment length was considered from the beginning of the primer sequence until the end of the promoter sequence.