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. Author manuscript; available in PMC: 2014 Sep 24.
Published in final edited form as: Immunity. 2013 Aug 15;39(2):357–371. doi: 10.1016/j.immuni.2013.07.018

Figure 4. Innate immune cells promote IL-33 dependent fibrosis.

Figure 4

(A) Il1rl1−/− or Il1rl1+/+ BM cells were i.v. injected into irradiated C57BL/6 recipient mice. 8 weeks later mice received mcIL-33 via HD injection. (B) Representative Sirius red stainings of livers of mice four weeks after treatment. (C) Hepatic collagen content was determined by hydroxyproline quantification (n=5/group; **p<0.01). (D, E, F) IL-33 expression constructs were HD injected into C57BL/6 or Rag1−/− mice. (D) Sirius red stainings of livers of mice 4 or 8 weeks after treatment. (E) Total collagen in livers was determined by hydroxyproline quantification (N=5-6 mice/group; *p<0.05). (F) Hepatic mRNAs of fibrosis-associated genes 4 weeks after mcIL-33 treatment in mice were quantified by qPCR (N=5/group; **p<0.01; *p<0.05). Data are representative of at least 2 different experiments with similar results. Scale bars 200μm.