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. 2014 Sep 23;5:4905. doi: 10.1038/ncomms5905

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(a) An arabinose-dependent promoter links the concentration-gradient input to a network of activators (T7 and SP6 RNAP) and repressors (lacI and TetR). (b) Each node is encoded on a different E. coli plasmid (color-coded). Each plasmid contains the same multiple cloning sites (MCS) for the combinatorial insertion of the network components. The plasmids contain compatible origins of replication (ori) and antibiotic resistance cassettes (kanamycin, spectinomycin and ampicillin resistance). The 3-node, 3-plasmid scaffold is described fully in Supplementary Methods.