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. 2014 Sep 15;28(18):2013–2026. doi: 10.1101/gad.246579.114

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© 2014 Boxer et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — Domains of ZNF750 required for protein–protein interactions and DNA binding. (A) Evolutionarily conserved C2H2 and PLNLS motifs in ZNF750 protein. (B) Diagram of mutations made in C2H2 and PLNLS motifs. (C) Inputs and Flag immunoprecipitation (IP) of tag-only control (CTL), full-length (FL), C2H2 mutant, or PLNLS mutant ZNF750 from keratinocytes, with Western blot for Flag, KLF4, RCOR1, KDM1A, CTBP1, or CTBP2. (D) ChIP-qPCR at ZNF750 target genes with HA-tagged full-length, C2H2 mutant, PLNLS mutant, or C2H2 PLNLS double-mutant ZNF750, compared with IgG controls. t-test: (*) P < 0.05; (**) P < 0.01, compared with full-length. Differentiation gene (E) or progenitor gene (F) expression changes with ectopic expression of full-length, C2H2 mutant, or PLNLS mutant ZNF750 in undifferentiated keratinocytes compared with tag-only control (CTL). (*) P < 0.05; (**) P < 0.01; (***) P < 0.001, compared with full-length) See also Supplemental Figure S4.

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