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. 2014 Sep 23;5:351. doi: 10.3389/fphys.2014.00351

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Copyright © 2014 Karunamuni, Gu, Ford, Peterson, Ma, Wang, Rollins, Jenkins and Watanabe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Anatomy of proximal and distal portions of the outflow tract in in cleared embryo hearts. OCT images in a plane transverse to the major great arteries of the heart in a stage 34/35 quail embryo allow the identification of the vessels. Comparison of OCT images from untreated (A) and ethanol-treated (B) embryos illustrates one of the post-septation stage consequences of ethanol treatment at gastrulation, a missing right pulmonary artery (RPA). The dimensions of these vessels such as wall thickness and diameters can be quantified using these OCT images. AA, aortic arch; LBA, left brachiocephalic artery; RBA, right brachiocephalic artery; LPA, left pulmonary artery; RPA, right pulmonary artery.