Figure 2.

B-cells proliferate in response to soluble or membrane-bound BAFF.

Notes: (A) T1165.17 cells were stimulated with a dose titration of membrane-bound BAFF (■), soluble BAFF (○), or vector control cells (▲). Proliferation was measured using a colorimetric method for determining the number of viable cells at 44 hours. (B) Human CD19⁺ B-cells were co-stimulated with a dose titration of membrane-bound BAFF (■), soluble BAFF (○), or vector control cells (▲) in the presence of anti-IgM and IL-4. Proliferation was measured using ³H-thymidine incorporation at 72 hours. Data are presented as mean ± SD.

Abbreviations: BAFF, B-cell activating factor; CPM, counts per minute; Ig, immunoglobulin; IL, interleukin; SD, standard deviation.