Figure 5.

Tabalumab prevents BAFF binding to the three BAFF receptors.

Notes: HEK293 cells were transfected with either empty vector (A), human BR3 (B), human TACI (C), or human BCMA (D). Tabalumab or isotype control was pre-incubated with soluble biotinylated BAFF for 15 minutes prior to addition to each cell line. BAFF binding is visualized by addition of streptavidin-PE and the fluorescence is read on a flow cytometer. Black solid line represents cells incubated with buffer only; dark gray histogram represents cells and isotype control antibody + biotinylated BAFF; dotted line with open histogram represents cells and tabalumab + biotinylated BAFF.

Abbreviation: BAFF, B-cell activating factor; PE, phycoerythrin.