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. 2014 Jan 16;63(10):1550–1559. doi: 10.1136/gutjnl-2013-306253

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This is an Open Access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 3.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited. See: http://creativecommons.org/licenses/by/3.0/

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Expression of homing receptors on CD4⁺CD25^hi events. Cells were isolated and stained for Treg markers and homing receptors. Example plots show CD4⁺ events with the gating used for (A) CCR6-A647 and CXCR2-A647 and (B) CXCR1-PE staining. The proportion of CD4⁺CD25^hi events expressing each receptor was determined using flow cytometry. (C) Peripheral blood mononuclear cells were analysed from 18 Helicobacter pylori-infected and 20 uninfected patients and (D) gastric biopsy samples from 18 H pylori-infected and 26 uninfected patients were analysed. (E) In addition, the total numbers of CD4⁺CD25^hiCCR6⁺ events isolated from gastric biopsy samples were calculated. p Values denote statistically significant differences.