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. 2014 Jan 16;63(10):1550–1559. doi: 10.1136/gutjnl-2013-306253

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This is an Open Access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 3.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited. See: http://creativecommons.org/licenses/by/3.0/

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Helicobacter pylori-dependent expression of CCL20 by epithelial cells in vitro after siRNA knockdown of signalling pathways. MKN28 cells were pretreated with siRNA duplexes in HiPerfect transfection reagent for 48 h. siRNA treatments targeted NF-κBp50 (NFKB1), NF-κBp65 (RELA) and MAPK1 genes. Negative controls were non-silencing duplexes; positive control duplexes targeted genes necessary for survival. Cells were then treated with 60190 H pylori for 24 h. (A) Western blot analysis showing knockdown by siRNA constructs. Supernatant CCL20 (B) and IL-8 (C) levels were then determined using ELISA. Graphs show means and SD from three independent experiments performed in triplicate. p Values show significant differences compared with H pylori-infected siRNA negative controls. MAPK, mitogen-activated protein kinase.